人、小鼠、大鼠常见细胞比例参考值—科研工具箱

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Knowing the frequency of cells in your flow assay is important to enable acquisition of sufficient cells.

These handy reference tables for human, mouse, and rat tissues, commonly used in flow cytometry, will help determine starting cell numbers for staining and cell sorting, allowing you to optimize your experiments.

了解流动分析中细胞的比例对于获取足够的细胞非常重要。

这些方便的人类,小鼠和大鼠组织参考表,通常用于流式细胞仪,将有助于确定染色和细胞分选的起始细胞数量,使您能够优化实验。

转载自Bio-Rad官网:flow-cytometry-cell-frequency.pdf

 

Table 1. Human cell frequency in peripheral blood.

Leukocytes

Lymphoid Cells

Myeloid Cells

Cell Type

Percent

Cell Type

Percent

Cell Type

Percent

T cells

10–25

CD4+

10–20

Neutrophils

40–65

B cells

3–10

CD8+

3–10

Eosinophils

0.1–2

Granulocytes

45–65

Tregs

0.2–1.4

Basophils

0.1–2

Monocytes

3–10

γδ T cells

1–5

Dendritic cells

0.5–1

NK cells

2–5

Naïve B cells

0.5–5

Monocyte DCs

0.2–0.4

Dendritic cells (DC)

0.5–1

Memory B cells

0.4–2

Plasmacytoid DCs

0.1–0.2

Stem cells

0.01–0.05

NKT cells

0.01–0.5

Innate lymphoid cells

0.01–0.1

Table 2. Mouse cell frequency.

Spleen

Thymus

Peripheral Blood

Lymph Node

Cell Type

Percent

Cell Type

Percent

Cell Type

Percent

Cell Type

Percent

T cells

21–35

CD4+

4–6

T cells

17–20

T cells

65–78

CD4+

13–20

CD8+

1–2

CD4+

8–12

CD4+

35–64

CD8+

7–15

CD4+/8+

85–95

CD8+

7–10

CD8+

19–30

γδ T cells

0.5–1

 

B cells

35–58

B cells

9–15

B cells

44–58

 

Monocytes

2–3

Tregs

2–3

Monocytes

3.5–5

 

Neutrophils

4–6

Dendritic cells

0.5–1

Granulocytes

1–2

 

Eosinophils

1–2

 

NK cells

1–5

 

NK cells

4–7

 

iNKT

1–2

 

iNKT cells

0.2–0.5

 

Myeloid DC

0.5–2

     

Lymphoid DC

0.5–1

     

Plasmacytoid DC

0.1–0.3

     

Table 3. Rat cell frequency.

Spleen

Thymus

Peripheral Blood

Cell Type

Percent

Cell Type

Percent

Cell Type

Percent

T cells

31.5–33.5

CD4+

5.7–9.4

T cells

51.5–66.5

CD4+

21.5–26.5

CD8+

2.3–6.9

CD4+

32–40

CD8+

14–18

CD4+/8+

83–87.6

CD8+

18–26

B cells

51–59

CD4-/8-

1.4–2.2

B cells

22.7–40.7

   

Monocytes

1–6

     

Neutrophils

14–20

   

Eosinophils

1–4

     

Basophils

Rare

   

γδ T cells

1–2

     

NK cells

6–10

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